Title / Titre :
Comparison of Leukocyte Reduction Efficiency and Residual Phenotypes in Blood Filtered with Two Different Leukocyte Reduction Filters
Author(s) / Auteur(s) :
Samuel O. Sowemimo-Coker, PhD
Address / Adresse :
Scientific and Laboratory Services, Pall Corporation, Port Washington, NY
Key words / Mots clés :
Abstract / Résumé : Background : Previous literature suggests that leukocyte associated transfusion reactions depend on the type and quantity of transfused white blood cells (WBC). Accordingly, to ensure the quality control of leukocyte reduced blood products it is important to use a technology that consistently meet or exceed FDA guidelines. We tested two different filters for their ability to effectively and consistently produce LR whole blood (WB) and defined phenotypic differences between the residual leukocyte populations produced.
Study Design and Methods : Sixteen units of 2-4 hour old CPD anticoagulated WB were studied. To create a homogenous challenge for each test, two units of ABO/Rh compatible WB were combined and redivided into equal aliquots of approximately 500mL each. Each aliquot was leukocyte reduced at 22±2ºC with either Pall Leukotrap® WBF-2 or Asahi Sepacell® RS2000. The residual WBC were quantified and phenotyped using previously published flow cytometric methods.
Results : Leukocyte residual counts in units filtered with the Pall filters ranged from 0.02 x 106 - 0.12 x 106 and from 2.06 x 106 - 11.7 x 106 for those filtered with Asahi RS-2000 filters. The data also demonstrate significant differences in these filters abilities to remove monocytes and B-lymphocytes from WB.
Conclusion : The present data confirm distinct differences in the leukocyte removal efficiency and residual white cell phenotypes obtained with the use of these filters. Such differences suggest that the clinical results produced by the transfusion of LR blood produced with different devices may not necessarily be identical which can only be ascertained by clinical study.